BS ISO 15213 pdf download

BS ISO 15213 pdf download Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of sulfite- reducing bacteria growing under anaerobic conditions
1 Scope
This International Standard specifies a horizontal method for the enumeration of sulfite-reducing bacteriagrowing under anaerobic conditions. It is applicable to
products intended for human consumption and the feeding of animals, and
environmental samples in the area of food production and food handling.
2 Normative references
The following referenced documents are indispensable for the application of this document. For datedreferences, only the edition cited applies. For undated references, the latest edition of the referenced document(including any amendments) applies.
SO 6887-1:1999,Microbiology of food and anima feeding stuffs — Preparation of test samples, initialsuspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation ofthe initial suspension and decimal dilutions
ISO 8261, Milk and milk products – General guidance for the preparation of test samples, initial suspensionsand decimal dilutions for microbiologicalexamination
ISO 7218:1996,Microbiology of food and anima feeding stuffs – General rules for microbiologicalexaminations
SO 7218:1996/Amd.1:2001, Microbiology of food and animal feeding stufs 一 General rules for microbiologicalexaminations-Amendment 1
ISO/TS 11133-1, Microbiology of food and animal feeding stuffs 一 Guidelines on preparation and production ofculture media — Part 1: General guidelines on quality assurance for the preparation of culture media in thelaboratory
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
sulfite-reducing bacteria growing under anaerobic conditionsbacteria forming countable typical colonies under the conditions specified in this lnternational Standard
4 Principle
4.1Two agar plates (or tubes) are prepared, using iron sulfite medium, and using a specified quantity of thetest sample if the initial product is liquid, or using a specified quantity of an initial suspension in the case of otherproducts.
5 Culture medium and diluent
For current practices, see ISO 7218.
5.1 Plate count medium: Iron sulfite agar
5.1.1 Composition
Enzymatic digest of casein 15 g
Pancreatic digest of soya 5 g
Yeast extract 5 g
5.1.2 Preparation
Dissolve the ingredients in the water by heating.
If necessary, adjust the pH so that after sterilization it is at .
7,6 ± 0,2 25 ◦ C
Pour 250 ml portions of the medium into 500 ml flasks.
If the enumeration is performed by use of tubes (6.5), pour 20 ml or 25 ml of medium into the tubes. Sterilize for in an autoclave set at .
15 min 121 ◦ C
Just before use, de-aerate the medium.
5.2 Saline peptone diluent
See ISO 6887-1:1999, 5.2.1.
6 Apparatus and glassware
Usual microbiological equipment (see ISO 7218) and, in particular, the following.
6.1 Homogenization equipment, for samples of solid food (see ISO 7218).